Journal: Oxidative Medicine and Cellular Longevity

Article Title: Intragastric Application of Aspirin, Clopidogrel, Cilostazol, and BPC 157 in Rats: Platelet Aggregation and Blood Clot

doi: 10.1155/2019/9084643

Figure Lengend Snippet: Rats which underwent antithrombotic agent aspirin (10 mg/kg intragastrically, once daily for three days) received immediately thereafter BPC 157 (10 μ g/kg intragastrically, once daily for three days) (white bars) or an equal volume of saline (5 ml/kg, intragastrically, once daily for three days) (gray bars); they were sacrificed at 2 h after the last application. Viscoelastic properties of the blood were assessed using modified rotational thromboelastometry (TEM) on ROTEM® delta analyzer (Tem International GmbH, Germany). Typical parameters obtained are clotting time (CT), the time from the beginning of measurement until the clot starts to form; clot formation time (CFT), the time needed for the clot to reach an amplitude of 20 mm; alpha-angle, angle of tangent at 2 mm amplitude; maximum clot firmness (MCF), the maximum amplitude of the curve during 60 minutes of measurement; Ly30, clot lysis at 30 minutes; and maximum lysis (ML) which describes the percentage of the maximum lost clot firmness relative to MCF. We analyzed the external pathway with tissue factor (EXTEM), an intrinsic pathway with ellagic acid (INTEM), or without platelet contribution with cytochalasin D (FIBTEM). After 60 minutes, CT, CFT, alpha-angle, MCF, Ly30, and ML were recorded. P > 0.05, vs. control.

Article Snippet: Viscoelastic properties of the blood were assessed using modified rotational thromboelastometry (TEM) on ROTEM® delta analyzer (Tem International GmbH, Germany).

Techniques: Modification, Coagulation, Lysis, FIBTEM Assay

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Intragastric Application of Aspirin, Clopidogrel, Cilostazol, and BPC 157 in Rats: Platelet Aggregation and Blood Clot

doi: 10.1155/2019/9084643

Figure Lengend Snippet: Rats which underwent antithrombotic agent cilostazol (10 mg/kg intragastrically, once daily for three days) received immediately thereafter BPC 157 (10 μ g/kg intragastrically, once daily for three days) (white bars) or an equal volume of saline (5 ml/kg, intragastrically, once daily for three days) (gray bars); they were sacrificed at 2 h after the last application. Viscoelastic properties of the blood were assessed using modified rotational thromboelastometry (TEM) on ROTEM® delta analyzer (Tem International GmbH, Germany). Typical parameters obtained are clotting time (CT), the time from the beginning of measurement until the clot starts to form; clot formation time (CFT), the time needed for the clot to reach an amplitude of 20 mm; alpha-angle, angle of tangent at 2 mm amplitude; maximum clot firmness (MCF), the maximum amplitude of the curve during 60 minutes of measurement; Ly30, clot lysis at 30 minutes; and maximum lysis (ML) which describes the percentage of the maximum lost clot firmness relative to MCF. We analyzed external pathway with tissue factor (EXTEM), an intrinsic pathway with ellagic acid (INTEM), or without platelet contribution with cytochalasin D (FIBTEM). After 60 minutes CT, CFT, alpha-angle, MCF, Ly30, and ML were recorded. P > 0.05, vs. control.

Article Snippet: Viscoelastic properties of the blood were assessed using modified rotational thromboelastometry (TEM) on ROTEM® delta analyzer (Tem International GmbH, Germany).

Techniques: Modification, Coagulation, Lysis, FIBTEM Assay

Journal: Frontiers in Physiology

Article Title: Adrenaline May Contribute to Prothrombotic Condition via Augmentation of Platelet Procoagulant Response, Enhancement of Fibrin Formation, and Attenuation of Fibrinolysis

doi: 10.3389/fphys.2021.657881

Figure Lengend Snippet: Effect of adrenaline on the kinetics of platelet-fibrin clot formation. Whole blood samples were incubated with or without rauwolscine (10 μM, for 10 min) followed by the addition of adrenaline (for 2 min) and collagen (150 ng/ml) with initial stirring (30 s). After 10 min of incubation, at room temperature, samples were analyzed toward kinetics of clot formation using rotational thromboelastometry. Clotting was initiated by recalcification (12 mM CaCl 2 final conc.). Control samples were without any addition. Representative coagulation profiles and records of clot formation rate (CFR) for 6 experiments are presented in (A) . (B–D) Parameters associated with clotting initiation (CT, clotting time; CFT, clot formation time), propagation (alpha angle; MaxV, maximal velocity of clot formation; MaxV-t, time to reach maximal velocity of clotting), and stabilization (MCF, maximum clot firmness; G, shear modulus strength) were measured. Data are means ± S.D. from 6 experiments. Parameters value range in control was: CT: 215–667 s; CFT: 79–176 s; Alpha: 64–74°; MaxV: 12–18 mm*100/s; MaxV-t: 351–855 s; MCF: 65–75 mm; G: 936–1,492. * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. control; # p < 0.05, ## p < 0.01, and ### p < 0.001 vs. adrenaline 10 nM + coll. (E) Whole blood samples (supplemented with AF488-fibrinogen to visualize fibrin formation) were incubated without any addition (control) or with adrenaline (for 2 min) or rauwolscine (10 μM, for 10 min) + adrenaline (for 2 min). Kinetics of fibrin formation within thrombi, formed under flow (1,000 s – 1 ) on collagen-coated surfaces, was recorded in confocal microscope. The fibrin(ogen)-associated fluorescence was recorded and expressed as% of maximal fluorescence obtained (considered as 100%) vs. time. Data are means ± S.D. from 4 experiments. * p < 0.05 vs. control; # p < 0.05 vs. adrenaline 1 nM.

Article Snippet: Thromboelastometric measurements were performed using ROTEM ® Delta rotational thromboelastometric system (Tem International GmbH, Manheim, Germany) ( ).

Techniques: Incubation, Coagulation, Microscopy, Fluorescence

Journal: Veterinary Sciences

Article Title: Improved Cardiovascular Tolerance to Hemorrhage after Oral Resveratrol Pretreatment in Dogs

doi: 10.3390/vetsci8070129

Figure Lengend Snippet: Rotational Thromboelastometry (ROTEM ® ) viscoelastic and thrombin generation parameters of the anesthetized greyhound dogs before and after induced hemorrhage ( n = 9).

Article Snippet: Physical examination, renal ultrasonography, urinalysis, complete blood count, serum creatinine (SCr), blood urea nitrogen, serum albumin concentration, platelet closure time (PCT), and Rotational Thromboelastometry (ROTEM ® delta, Tem International GmbH, Munich, Germany) for all dogs were within reference intervals for adult Greyhounds [ ].

Techniques:

Journal: Veterinary Sciences

Article Title: Improved Cardiovascular Tolerance to Hemorrhage after Oral Resveratrol Pretreatment in Dogs

doi: 10.3390/vetsci8070129

Figure Lengend Snippet: Linear mixed models showing the associations between resveratrol treatment with maximum clot firmness on the Rotational Thromboelastometry (ROTEM ® ) of the anesthetized greyhound dogs before and after induced hemorrhage ( n = 12) after adjusting for volume of blood removed using unstructured covariance structure. MCF = maximum clot firmness.

Article Snippet: Physical examination, renal ultrasonography, urinalysis, complete blood count, serum creatinine (SCr), blood urea nitrogen, serum albumin concentration, platelet closure time (PCT), and Rotational Thromboelastometry (ROTEM ® delta, Tem International GmbH, Munich, Germany) for all dogs were within reference intervals for adult Greyhounds [ ].

Techniques:

Journal: Journal of thrombosis and haemostasis : JTH

Article Title: Abrogating fibrinolysis does not improve bleeding or rFVIIa/rFVIII treatment in a non-mucosal venous injury model in haemophilic rodents

doi: 10.1111/jth.14148

Figure Lengend Snippet: Schematic depiction and representative examples of rotational thromboelastometry (ROTEM) traces recorded during recalcification, tissue factor (TF) and TF/tissue-type plasminogen activator (t-PA) ROTEM assays with whole blood from treated FVIII−/− rats. (A) Schematic depiction of a ROTEM curve that demonstrates where clot initiation, propagation, stabilization, lysis and dissolution of a formed thrombus can be detected. (B) Representative examples of recorded ROTEM traces from the recalcified whole blood assay, which assesses global clot formation. (C) Representative examples of recorded ROTEM traces for the TF assay, which assesses global clot formation following activation of coagulation with TF. (D) Representative examples of ROTEM traces for the TF/t-PA assay, which assesses the formation and stability of clots following addition of t-PA to all blood samples. Each trace in (B), (C) and (D) shows the rat whole blood sample that best represented its treatment group on the basis of mean clotting time and maximum clot firmness results. rFVIIa, recombinant activated factor VII; TXA, tranexamic acid; WT, wild-type.

Article Snippet: Whole blood coagulation profiles were obtained by use of a ROTEM delta hemostasis analyzer (TEM International, Munich, Germany) and prewarmed plastic test cups.

Techniques: Lysis, Whole Blood Assay, Transcription Factor Assay, Activation Assay, Coagulation, Recombinant